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Expression analysis of gp120. In vitro expression of gp120 protein from L. lactis (pLL120) and E. coli (pEC120) plasmids with and without CpG motifs. RIPA of culture supernatants of 35S-cys/met-pulsed HEK-293 cells transfected with the vaccine plasmids. Precipitation is done using pooled serum from HIV positive patients and the immunoprecipitates were analysed by SDS-PAGE. Positive control plasmid (Pos), untransfected cells (Neg), molecular marker (Marker). The gp120 is indicated.
