Skip to main content
. Author manuscript; available in PMC: 2007 Feb 2.
Published in final edited form as: Nat Struct Mol Biol. 2005 Nov 6;12(12):1086–1093. doi: 10.1038/nsmb1015

Figure 4.

Figure 4

Tgt inhibits all catalytic reactions. (a,b) Pyrophosphorolysis and GreA-enhanced transcript cleavage assays. Halted radiolabeled A26 TECs were purified from NTPs using G50 spin columns and pre-incubated with Tgt at 37 °C for 2 min. GreA (300 nM) or PPi (400 μM) were added at time 0, followed by incubation at 37 °C. (c) Exonuclease cleavage assay. 3′ radiolabeled C27 complexes were incubated with water (left panel), 2 μM Tgt (center), or 1 mM ATP (right panel) at 37 °C. Aliquots were removed at the indicated times and quenched.