Table 1.
Data collection and refinement statistics
| ttRNAP/Tgt | |
|---|---|
| Data collection | |
| Space group | P65 |
| Cell dimensions | |
| a, b, c (Å) | 239.5, 239.5, 253.1 |
| α, β, γ (°) | 90.0, 90.0, 120.0 |
| Resolution (Å) | 25.0 – 2.4(2.49-2.4)* |
| R merge | 0.105(0.425) |
| I / σI | 10.2(2.3) |
| Completeness (%) | 95.1(93.4) |
| Redundancy | 2.9(2.5) |
| Refinement | |
| Space group | P32# |
| Resolution (Å) | 25.0 - 2.4 |
| No. reflections | 604295 |
| Rwork / Rfree | 23.8/27.3 |
| No. atoms | |
| Protein | 53574 |
| Tgt | 52 |
| Mg2+ ions | 4 |
| Zn2+ ions | 4 |
| Water | 8187 |
| B-factors | |
| Protein | 55.7 |
| Tgt | 45.0 |
| Mg2+ ions | 11.5 |
| Zn2+ ions | 46.1 |
| Water | 34.6 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.015 |
| Bond angles (°) | 1.890 |
*
Highest resolution shell is shown in parenthesis.
#
The crystals belong to P32 space group with a perfect (50%) merohedral twinning mimicking P65 space group. The data were therefore processed in P65 space group and were expanded to P32 space group for refinement.