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. 2007 Feb 6;5(2):e40. doi: 10.1371/journal.pbio.0050040

Figure 5. Degenerate Sequences Can Act as CaRRE 1 and CaRRE 2.

Figure 5

(A) and (B) Top: single nucleotide scanning mutagenesis of CaRRE 1 and CaRRE 2. The ApaI site of pDup175 was mutated to XhoI site to facilitate the cloning. Each nucleotide of CaRRE 1 (A) and CaRRE 2 (B) was mutated to the other three possible nucleotides, one at a time. Each mutant was co-transfected with CaMK IV-dCTK75E (m) or CaMK IV-dCT (IV) Bottom right of (A) and (B): summary of the relative activity of the CaRRE mutants. The activities of wild-type CaRREs were defined as 100%, and the relative activity of each mutant was calculated by normalizing the difference of the percentage of exon inclusion caused by CaMK IVdCT to the difference observed in its wild-type CaRRE. These mutants are classified by their relative activities (left column). The 8A to 8C mutation in CaRRE 2 disrupted the splicing of this minigene, and the relative exon inclusion could not be determined.