Fig. 4.

Quantitative measurement of the abundance of the detected sequences by real time PCR. The same sequences, primers and RNA samples used in Figure 2 were used for this analysis. See supplementary table 1 for detailed sequence information. (No. 2 sequence DR978181 was not used for this analysis, as its melting temperature is only 53°C, far lower than the 60°C recommended for real-time PCR). The beta-actin transcripts were used as positive control. A. Histogram of real-time PCR showing the amplification dynamics for each detected sequence. B. Relative abundance of each sequence normalized to beta-actin. Note that the weakly amplified templates by regular PCR in Figure 4 were well reflected by their lower abundance detected by real time PCR.