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. 2007 Feb;8(2):136–146. doi: 10.1631/jzus.2007.B0136

Fig. 4.

Fig. 4

Fig. 4

Fig. 4

Fig. 4

Fig. 4

Fig. 4

Fig. 4

The differentiation of resuscitated hMSCs into neurocytes. (a) Nestin staining of cells from passage 15 of post-cryopreservation induced for 1 d (×100); (b) NSE staining of cells from passage 15 of post-cryopreservation induced for 6 d (×250); (c) NSE staining of cells from passage 15 of post-cryopreservation induced for 6 d (×400); (d) RT-PCR of nestin mRNA in cells induced for 1 d; (e) RT-PCR of NSE mRNA in cells induced for 6 d; (f) Western blotting of nestin in cells induced for 1 d; (g) Western blotting of NSE in cells induced for 6 d

(d)~(f) Line 1: induced cells from passage 15 of post-cryopreservation, line 2: induced cells from passage 3 of pre-cryopreservation, line 3: uninduced cells from passage 15 of post-cryopreservation; (g) Line 1: uninduced cells from passage 15 of post-cryopreservation, line 2: induced cells from passage 15 of post-cryopreservation, line 3: induced cells from passage 3 of pre-cryopreservation