Table 1.
Passage | CD34** | CD45 | CD29 | CD44 | CD90 | CD166 | SH2 | SH3 |
Pre-3* | 3.8±5.2a | 5.2±4.7a | 30.2±11.2a | 29.6±7.8a | 37.6±19.2a | 47.5±6.9a | 37.8±5.7a | 27.2±15.2a |
Pre-4 | 3.8±4.3a | 5.1±3.3a | 24.8±9.2a | 27.4±11.4a | 35.6±10.5a | 48.6±16.2a | 35.8±8.9a | 41.2±14.2a |
Pre-8 | 2.0±0.9 | 3.3±1.1b | 57.3±9.4a | 43.5±12.2a | 59.8±12.7b | 75.4±9.5b | 60.4±7.9a | 55.5±11.2a |
Pre-13 | 1.2±0.5 | 0.9±0.3 | 75.2±11.6 | 81.4±15.7 | 70.3±14.4 | 81.5±16.6 | 83.7±18.2 | 80.9±12.4 |
Pre-18 | 1.1±0.7 | 0.5±0.3 | 82.6±10.4 | 85.8±15.8 | 80.2±18.6 | 90.4±14.5 | 84.2±14.2 | 82.4±10.8 |
Post-1 | 3.3±2.5a | 5.9±6.2a | 27.3±13.0a | 29.1±17.2a | 34.7±9.9a | 50.8±14.6a | 41.4±9.3a | 39.4±11.7a |
Post-5 | 1.5±0.7 | 3.6±1.3a | 54.8±8.2a | 46.4±13.3a | 57.2±15.4b | 79.5±12.8b | 62.2±10.2a | 53.9±7.5a |
Post-10 | 1.7±0.9 | 1.1±0.6 | 73.3±16.1 | 79.6±18.7 | 68.8±17.3 | 84.5±20.2 | 86.4±6.8 | 82.5±9.3 |
Post-15 | 0.9±1.2 | 0.2±0.5 | 87.4±7.7 | 88.3±11.5 | 81.7±12.5 | 93.5±10.4 | 80.1±9.5 | 79.3±10.4 |
Pre-: Pre-cryopreservation; Post-: Post-cryopreservation
CD34: Hematopoietic stem cell marker; CD45: Leucocyte common antigen (LCA); CD29: Beta-1 integrin; CD44: Family of cell surface glycoproteins; CD90: Thy-1; CD166: Activated leukocyte cell adhesion molecule (ALCAM); SH2: An Src-homology 2 domain; SH3: An Src-homology 3 domain. CD34−, CD45−, CD29+, CD44+, CD90+, CD166+, SH2+ and SH3+ are immunophenotypic properties of hMSCs (Pittenger et al., 1999)
Comparison with Post-10 passage: P<0.01
Comparison with Post-10 passage: P<0.05