Abstract
In this study a second endogenous Porphyromonas gingivalis insertion element (IS element) that is capable of transposition within P. gingivalis was identified. Nucleotide sequence analysis of the Tn4351 insertion site in a P. gingivalis Tn4351-generated transconjugant showed that a complete copy of the previously unidentified IS element, designated PGIS2, had inserted into IS4351R in Tn4351. PGIS2 is 1,207 bp in length with 19-bp imperfect terminal inverted repeats, and insertion resulted in a duplicated 10-bp target sequence. Results of Southern hybridization of chromosomal DNA isolated from several P. gingivalis strains with a PGIS2-specific probe demonstrated that the number of copies of PGIS2 per genome varies among different P. gingivalis strains. Computer analysis of the putative polypeptide encoded by PGIS2 revealed strong homologies to the products encoded by IS1358 from Vibrio cholerae, ISAS1 from Aeromonas salmonicida, and H-rpt in Escherichia coli K-12.
Full Text
The Full Text of this article is available as a PDF (474.4 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Genco C. A., Schifferle R. E., Njoroge T., Forng R. Y., Cutler C. W. Resistance of a Tn4351-generated polysaccharide mutant of Porphyromonas gingivalis to polymorphonuclear leukocyte killing. Infect Immun. 1995 Feb;63(2):393–401. doi: 10.1128/iai.63.2.393-401.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Genco C. A., Simpson W., Forng R. Y., Egal M., Odusanya B. M. Characterization of a Tn4351-generated hemin uptake mutant of Porphyromonas gingivalis: evidence for the coordinate regulation of virulence factors by hemin. Infect Immun. 1995 Jul;63(7):2459–2466. doi: 10.1128/iai.63.7.2459-2466.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gustafson C. E., Chu S., Trust T. J. Mutagenesis of the paracrystalline surface protein array of Aeromonas salmonicida by endogenous insertion elements. J Mol Biol. 1994 Apr 8;237(4):452–463. doi: 10.1006/jmbi.1994.1247. [DOI] [PubMed] [Google Scholar]
- Hallet B., Rezsöhazy R., Mahillon J., Delcour J. IS231A insertion specificity: consensus sequence and DNA bending at the target site. Mol Microbiol. 1994 Oct;14(1):131–139. doi: 10.1111/j.1365-2958.1994.tb01273.x. [DOI] [PubMed] [Google Scholar]
- Hilse R., Hammerschmidt S., Bautsch W., Frosch M. Site-specific insertion of IS1301 and distribution in Neisseria meningitidis strains. J Bacteriol. 1996 May;178(9):2527–2532. doi: 10.1128/jb.178.9.2527-2532.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hwa V., Shoemaker N. B., Salyers A. A. Direct repeats flanking the Bacteroides transposon Tn4351 are insertion sequence elements. J Bacteriol. 1988 Jan;170(1):449–451. doi: 10.1128/jb.170.1.449-451.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Janssen D. B., Pries F., van der Ploeg J., Kazemier B., Terpstra P., Witholt B. Cloning of 1,2-dichloroethane degradation genes of Xanthobacter autotrophicus GJ10 and expression and sequencing of the dhlA gene. J Bacteriol. 1989 Dec;171(12):6791–6799. doi: 10.1128/jb.171.12.6791-6799.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maley J., Roberts I. S. Characterisation of IS1126 from Porphyromonas gingivalis W83: a new member of the IS4 family of insertion sequence elements. FEMS Microbiol Lett. 1994 Oct 15;123(1-2):219–224. doi: 10.1111/j.1574-6968.1994.tb07225.x. [DOI] [PubMed] [Google Scholar]
- Rasmussen J. L., Odelson D. A., Macrina F. L. Complete nucleotide sequence of insertion element IS4351 from Bacteroides fragilis. J Bacteriol. 1987 Aug;169(8):3573–3580. doi: 10.1128/jb.169.8.3573-3580.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stroeher U. H., Jedani K. E., Dredge B. K., Morona R., Brown M. H., Karageorgos L. E., Albert M. J., Manning P. A. Genetic rearrangements in the rfb regions of Vibrio cholerae O1 and O139. Proc Natl Acad Sci U S A. 1995 Oct 24;92(22):10374–10378. doi: 10.1073/pnas.92.22.10374. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zhao S., Sandt C. H., Feulner G., Vlazny D. A., Gray J. A., Hill C. W. Rhs elements of Escherichia coli K-12: complex composites of shared and unique components that have different evolutionary histories. J Bacteriol. 1993 May;175(10):2799–2808. doi: 10.1128/jb.175.10.2799-2808.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]