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RNA blot showing Incw1, Sh1, and Sus1 transcripts in 20 μg of total RNA at various time points (hours). (A) Suc+ medium. (B) Fresh suc-deficient medium. (C) Fresh suc-deficient medium at 0 and 48 hr, thereafter in the suc+ medium as shown. (D) Mannitol (M) or suc (S) is added after a 48-hr preculture in suc-deficient medium for the duration as shown. For all treatments, 0 hr represents 7-day cultured cells that were washed three times in suc-deficient medium.
