(A) RNA blot showing Incw1, Sh1, and Sus1 transcripts in 20 μg of total RNA after incubation for 0 or 48 hr in suc-deficient medium (−Suc; first two lanes and last lane) or after incubation in suc-deficient medium for 48 hr followed by addition of water (−Suc), +Suc, +d-Glc, 3-O-mGlc (+3OMG), +l-Glc, or mannitol (+Man) for 12 or 24 hr. (B) SDS Western blot showing INCW1, SS1, and SS2 proteins in crude extracts of cells in the media as above. Each lane contains 50, 10, and 5 μg of protein for INCW1, SS1, and SS2 proteins, respectively.