Table 1.
Culture | Invertase specific activity (%) |
---|---|
Suc-deficient | |
0 hr | 0.770 ± 0.0054 (100) |
12 hr | 0.519 ± 0.042 (67.4) |
24 hr | 0.415 ± 0.058 (53.9) |
48 hr | 0.340 ± 0.035 (44.2) |
72 hr | 0.229 ± 0.011 (29.7) |
Suc-deficient for 48 hr followed by 12 hr with sugar | |
+suc | 0.576 ± 0.014 (74.8) |
+D-Glc | 0.507 ± 0.036 (65.8) |
+3-O-mGlc | 0.209 ± 0.041 (27.1) |
+2-dGlc | 0.292 ± 0.022 (37.9) |
Values are means ± SD of three independent experiments (except for +2DG, for which the value represents the mean ± SD of two experiments); 0 hr represents a 7-day-old culture washed three times with suc-free medium. D-Glc, d-glucose.
Micromoles of reducing sugars per milligram of protein per minute.