Figure 2.

Description of the plasmids and oligonucleotides used in this study. Plasmid pAR1 was constructed by insertion of the androgen receptor promoter (−546, +131) between the BamHI and XhoI sites of the pBL-CAT plasmid (17). The sequence corresponding to positions −119 to −70 is indicated. The target sequence for triple helix formation is located between positions −102 and −88 on the promoter sequence. Sequences of the 89-mer oligonucleotide and the 20-mer template are shown. The 89-mer can form a triple helix by binding to a 15-bp sequence target in the pAR1 plasmid. In the pAR4 plasmid, the G⋅C base pairs indicated by an asterisk were mutated to T⋅A base pairs. Position of the restriction site for BsiWI within the 20-bp double helix is also indicated.