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. 1999 Sep 14;96(19):10620–10625. doi: 10.1073/pnas.96.19.10620

Figure 2.

Figure 2

(A) Inhibition of PP1/PP2A induces rapid (<1 hr) serine phosphorylation of STAT3. T lymphoma cells were incubated with CA (80 nM) or CyA (400 ng/ml) for 5–60 min, lysed, applied to SDS/PAGE, and immunoblotted with anti-STAT3 pS 727 pAb, stripped and reblotted with anti-STAT3 (S21320), anti-STAT3 (K-15), and anti-STAT5. (B) PP2A-specific, but not a PP1-specific, inhibitor, induced STAT3 serine phosphorylation. T lymphoma cells were incubated in either TAU (500 nM), OA (500 nM), ETA (100 μM), CA (80 nM), or CyA (400 ng/ml) for 60 min, lysed in lysis buffer and applied to SDS-PAGE, and immunoblotted with anti-STAT3 pS727 (Upper) and stripped, and reblotted with anti-STAT3 (Lower).