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. 1999 Sep 14;96(19):10620–10625. doi: 10.1073/pnas.96.19.10620

Figure 5.

Figure 5

Inhibition of PP2A modulates the subcellular distribution of STAT3. (A) Confocal laser scanning microscopy analysis of STAT3 location in T lymphoma cells incubated with solvent (media, Top), 80 nM CA (Middle), or 400 ng/ml CyA (Bottom). Cells were fixed in 1% paraformaldehyde in PBS and stained with anti-STAT3 (#9132) followed by confocal laser scanning microscopy analysis as described in Materials and Methods. (Right) Green fluorescence. (Center) Blue interference contrast. (Left) combination of both. (B) Serine phosphorylation inhibits nuclear translocation of STAT3. Cytoplasmic and nuclear extracts from T lymphoma cells incubated in medium CA (80 nM) or CyA (400 ng/ml) for 60 min were prepared as described in Materials and Methods and subsequently immunoblotted with anti-STAT3 pS727 (Upper) and stripped and reblotted with anti-STAT3 (Lower).