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. 1999 Sep 14;96(19):10626–10631. doi: 10.1073/pnas.96.19.10626

Figure 5.

Figure 5

(A) Effect of Gβγ subunit expression on signaling by wild-type B2 receptors and the B22 fusion protein. The Gβ1γ2-mediated enhancement of the bradykinin-stimulated signal (100 nM) was determined on COS cells expressing the B2 receptor (■) or the B22 fusion protein (░⃞) together with Gβ1γ2. The enhancement is given as percent above control cells (0%): i.e., cells transfected with wild-type B2 receptor or with B22 fusion protein alone. (B and C) Suppression of crosstalk with Gαi-coupled receptors in a B22 fusion protein. COS cells expressing the B2 receptor (■) or the B22 fusion protein (░⃞) together with the A1 adenosine (B) or the α2C-adrenergic (C) receptor were stimulated with 100 nM bradykinin plus 1 μM N6-cyclohexyladenosine in the presence of 0.5 units/ml adenosine deaminase (B) or plus 1 μM medetomidine (C). Expression levels of the transfected B2 receptors were 3.3 ± 0.4 pmol/mg protein. Expression levels of the transfected A1 and α2C receptor were 2.1 ± 0.4 and 1.8 ± 0.3 pmol/mg protein, respectively. Data are given as percent above control (0%): i.e., stimulation with bradykinin alone. All data are the means (±SEM) of three different experiments.