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Polysome association in resting and activated human foreskin fibroblasts of mRNAs encoding vimentin, 23-kDa highly basic protein and β-actin. Cells were growth-arrested by serum starvation and stimulated by addition of serum (18). Cytoplasmic extracts were prepared and separated on sucrose gradients (9). (Upper) Optical density profiles of sucrose gradient. (Lower) Northern blot analysis of the RNA extracted from sucrose gradient fractions.
