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In vitro splicing kinetics of IgM pre-mRNA. A time course of cis-splicing of IgM pre-mRNAs with (IgM-SF2W) or without (μMΔ) an SF2/ASF-specific ESE is shown. The positions of the pre-mRNA, 5′ exon M1 and lariat-exon M2 intermediates, and splicing products are shown by diagrams on the right. A dashed arrow shows the expected position of the μMΔ-spliced product. Lane 1, pBR322/MspI size markers; lanes 7 and 13, control reactions lacking ATP.
