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SR protein specificity of second-step trans-splicing enhancement. (A) 32P-labeled AdML ΔAG pre-mRNA was incubated for 1 hr in S100 extract (with or without the indicated SR proteins), and 3H-labeled M2-SF2W 3′ exon was added for the second hour. Trans-spliced mRNA is shown by the black arrow. Lane 1: size markers. (B) A reciprocal labeling experiment is shown. The top part of the autoradiogram is a longer exposure. Lane 1, size markers.
