ATP dependence of ESE-stimulated bimolecular exon ligation. Tritiated AdML ΔAG RNA was incubated for 1 hr (except in lane 2), followed by glucose (glu) and hexokinase (hex; lane 3), or control treatments (lanes 3, 5, and 6), with subsequent addition of radiolabeled M2-SF2W 3′ RNA. The trans-spliced product (TS) is marked by an arrow. Lane 1, size markers.