Figure 6.

Susceptibility of the mel2 mutant to reactive nitrogen species. Susceptibility of M. marinum wild type as compared to the mel2 mutant that carries an insertion in melF (melF), the mutant carrying a plasmid expressing melF alone (melF::pJDC79) and an integrated single copy plasmid expressing the entire mel2 locus (melF::pJDC75) to 1 mM (A) or 5 mM (B) sodium nitrite (NaNO₂) and 10 μM (C) or 100 μM (D) S-nitroso-N-acetyl penicillamine (SNAP). The % survival = (CFU Tx/CFU initial) × 100. Data are the means and standard deviations of assays done in triplicate, representative of three experiments.