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(a) RepA activation by Hsp104 and mutants in NBD-1 and NBD-2. Data are means ± SD (n=3).
(b) Reactivation of heat-aggregated GFP by Hsp104 mutants. The initial fluorescence was set equal to 0 and a representative experiment is shown of three or more replicates.
(c) Unfolding of RepA(1–70)-GFP by Hsp104 mutants. The initial fluorescence was set equal to 1 and a representative experiment is shown of three or more replicates.
