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. 2007 Jan 29;104(6):1811–1816. doi: 10.1073/pnas.0607216104

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© 2007 by The National Academy of Sciences of the USA

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Fig. 4. — ParGR19K and ParGR19A are as proficient as wild-type ParG in stimulating ParF filamentation. (A) DLS experiment in which ParG, ParGR19K, or ParGR19A was added to polymerizing ParF (2.16 μM) at a molar ratio of 10:1 (ParF/ParG). (B) Sedimentation assay. ParF (10 μM) was incubated without nucleotide, with ATP (2 mM) only, or with ParG, ParGR19K, or ParGR19A (10 μM). Reactions were centrifuged, and 100% of pellet (P) and 33% of supernatant (S) were resolved on a 15% SDS gel. Percentages of proteins in the pellet are shown.