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Effects of LIF and EGF on the proliferation of chromaffin cells from young (A) or adult (B) cattle. Cells were cultured for 96 h in DMEM/F12 containing the indicated growth factors. Cell proliferation was then assessed with MTS reagent and measurement of absorbance at 490 nm. The results are expressed as the mean ± SEM of three to six independent experiments. ∗∗∗, P < 0.001 vs. control.
