Fig. 7.
Effect of 10−5 M DHEAS on 200 ng/ml EGF-induced chromaffin cell proliferation after 96 h of pharmacological inhibition of androgen receptor by flutamide (A), estrogen receptor by ICI 182,780 (B), and GABAA receptor by SR 95531(C). Cell proliferation was assessed with MTS reagent and measurement of absorbance at 490 nm. The results are expressed as the mean ± SEM of three to eight independent experiments. ∗∗∗, P < 0.001 vs. control; #, P < 0.05; ##, P < 0.01; ###, P < 0.001 vs. response to EGF.