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Steady-state kinetic parameters of HsaCH37Rv and HsaDH37Rv for steroid and biphenyl metabolites
| Enzyme | Substrate | Km, μM | Vmax, μM·s−1 | Vmax/ Km, s−1 |
|---|---|---|---|---|
| HsaCH37Rv | 3,4-DHSA | 0.9 (0.5) | 12 (4) | 790 (370) |
| DHB | 8.5 (0.8) | 2.5 (0.4) | 18 (3) | |
| HsaDH37Rv | 4,9-DSHA | 4 (1) | 0.06 (0.02) | 1.0 (0.2) |
| HOPDA | 19 (6) | 0.009 (0.003) | 0.028 (0.007) |
Parameters were normalized to the amount of cellular extract (milligrams of protein content) used in the assays. Values in parentheses represent standard errors.
