Fig. 3.

Amino acid uptake into Atoep16-1 and wild-type chloroplasts. (A) Isolated chloroplasts were incubated with ¹⁴C-glutamate for various time intervals, and uptake of radioactivity was determined. (B) After labeling isolated chloroplasts with [³⁵S]methionine for 5 min (lanes 1 and 2) or 20 min (data not shown), protein was extracted and precipitated with trichloroacetic acid, separated by SDS/PAGE, and detected by either autoradiography (lanes 1 and 2) or Coomassie staining (lanes 3 and 4). LSU and SSU, large and small subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase; CAB, chlorophyll a/b-binding protein of photosystem II.