Fig. 3.

Assessment of phospho-histone H3 in xenografted tumors. Nude mice were injected at an s.c. site with 10⁷ PC3 cells in the left flank. Eight days later, the mice were randomized into three groups of two mice each and treated with vehicle control (lanes 1 and 2), 20 mg/kg paclitaxel (lanes 3 and 4), and 20 mg/kg AB-5 (lanes 5 and 6). The mice were killed 12 h later, and extracts from the tumors were immunoblotted with antiphosphorylated histone H3 and antiactin antibodies.