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. 2007 Feb 7;104(7):2199–2204. doi: 10.1073/pnas.0611217104

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© 2007 by The National Academy of Sciences of the USA

Freely available online through the PNAS open access option.

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Fig. 1. — A schematic depicting the strategy for analysis of phosphopeptides carried out in this study. All fractions were analyzed by LC-MS/MS. A total of 30 Lys-C digested fractions were analyzed by LC-MS/MS (ETD); 12 of the selected Lys-C fractions (1/3 of each sample was used) were subjected to a replicate analysis, with one part of a sample analyzed by ETD and the other by CID; the remaining 2/3 of the 12 protein fractions were digested by using trypsin or Glu-C, respectively, and analyzed in replicate by LC-MS/MS (CID) and LC-MS/MS (ETD). A fraction (7%) of the digested samples before enrichment of phosphopeptides was also analyzed by CID.