Figure 6.

Splicing and cropping of four exons–three introns reporters. (A) Schematic depiction of the constructs used in this experiment. The hairpin represents the miR-26b stem–loop and the arrows indicate the position of primers. The mature miRNA sequences are written in bold whereas the mutated sequences are shown in red. (B) Northern blot analysis of miR-26b reporter expression. Two days after transfection into HEK293T cells, total RNA was extracted and analyzed by Northern blotting. (C) Time-course experiment of the reporter constructs. After transfection of the reporter plasmid into HEK293T cells, total RNA was prepared at the indicated time. The neomycin resistance gene was used as transfection efficiency control. All experiments were performed at least three times and representative results are shown.