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. 2007 Jan 25;26(3):855–866. doi: 10.1038/sj.emboj.7601528

Figure 1.

Figure 1

Post-translational acquisition of WT-SOD1 resistance to proteinase K does not require ATP. (A) Schematic diagram of synchronized cell-free translation assay. (B) Kinetics of acquisition of resistance to 0.01 mg/ml proteinase K. Translations were terminated at the indicated times and radioactivity incorporated into SOD1 was assessed by SDS–PAGE/phosphorimage analysis before (−) or after (+) digestion with 0.01 mg/ml proteinase K (top panel). Normalized data (mean±s.e.m.) from multiple experiments (n=3–12) were fitted using least squares regression (bottom panel). (C) Kinetics of acquisition of resistance to 1 mg/ml proteinase K. Samples were analyzed and presented as in (B).