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. 2007 Jan 25;26(3):855–866. doi: 10.1038/sj.emboj.7601528

Figure 3.

Figure 3

WT-SOD1 dimerization is not required for proteinase K resistance. (A) Representative gel filtration profiles for WT-SOD1 and the monomeric variant F50E/G51E. (B) Kinetics of WT-SOD1 dimerization. (C, D) Kinetics of acquisition of resistance to 0.01 mg/ml (C) and 1 mg/ml (D) proteinase K for the F50E/G51E variant compared to WT-SOD1 (from Figure 1). Samples were prepared and analyzed as in Figure 1B. (E) Quantification of the oxidized fraction of F50E/G51E as a function of time. Inset, initial rates of disulfide bond formation for WT-SOD1 and F50E/G51E fitted to linear least-squares regression model. Graphical data are mean±s.e.m. from a minimum of three independent experiments per time point.