Figure 4.

Effects of FALS-linked metal-binding loop mutations on SOD1 folding. Kinetics of acquisition of resistance of metal-binding FALS mutants to 0.01 mg/ml (A) and 1 mg/ml (B) proteinase K. Inset, initial rates of protease resistance for WT-SOD1 and mutants fitted to a linear least-squares regression model. (C) Kinetics of disulfide bond formation as described in Figure 2C. (D) Gel filtration profile for H46R as in Figure 3A. (E) Quantification of H46R dimerization kinetics compared with WT-SOD1 data from Figure 3B. Graphical data are mean±s.e.m. from a minimum of three independent experiments per time point.