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. 2007 Jan 25;26(3):647–656. doi: 10.1038/sj.emboj.7601534

Figure 6.

Figure 6

Requirement for CIN85, Cbl and binding of CIN85 with AMAP1 in the invasion of different breast cancer cells. (A) Protein expression of CIN85, c-Cbl and Cbl-b. Total cell lysates (10 μg) were subjected to immunoblotting analysis using antibodies, as indicated. Invasion and chemotactic activities from the literature are shown below, in which activities graded as % MDA-MB-231; L, 0–40%; M, 40–60%; and H, more than 60%. (B) Effects of knockdown of CIN85 and Cbl on Matrigel chemoinvasion. Cells were treated with siRNA duplexes for CIN85 or Cbl (both c-Cbl and Cbl-b), or with an irrelevant sequence (irr), and cultured on Biocoat Matrigel chambers. (C) Effects of P17-TAT. Cells were cultured on Biocoat Matrigel chambers in the presence of 250 μM of P17-TAT or scramble TAT peptide. In (B) and (C) data collection and presentation are the same as in Figure 1C and F, respectively. Results represent the mean±s.e. of three independent experiments. In (B) the upper panels show protein expression in these cells by immunoblotting.