Figure 2.

Induction of cell-cycle-specific proteins in the knockdown lines. Cell cultures, serum-depleted and re-stimulated as described in Figure 1, were harvested at hourly intervals up to 12 h post-stimulation, and at two hourly intervals thereafter, as indicated above the panels. Lysates were analyzed by Western blot for c-Myc (A), cdc2 (B), cyclin E (C), and cyclin A (D). For each protein, accumulation is accelerated in p270/ARID1A-depleted cells and delayed in ARID1B-depleted cells, consistent with the DNA synthesis kinetics seen in Figure 1.