Table 2.
Medaka mutant library* screening statistics
| Gene | Exons | Exons screened | Amplicons† | Base-pairs screened‡ | Exonic | Intronic | Total | Mutation rate | |||
| Stop | Missense | Silent | Intron | Splice | |||||||
| Blm | 23 | 2 | 2 | 3,129,006 | 1 | 4 | 0 | 1 | 0 | 6 | 1/521,501 |
| p53 | 11 | 3 | 1 | 1,854,603 | 2 | 3 | 0 | 1 | 1 | 7 | 1/264,943 |
| Sirt1 | 9 | 5 | 2 | 5,767,496 | 0 | 12 | 0 | 2 | 1 | 15 | 1/384,500 |
| Dcr-1 | 27 | 7 | 4 | 7,879,290 | 0 | 16 | 4 | 7 | 0 | 27 | 1/291,826 |
| Parkin | 11 | 3 | 1 | 3,461,661 | 1 | 3 | 3 | 2 | 0 | 9 | 1/384,629 |
| Total | 81 | 20 | 10 | 22,092,056 | 4 | 38 | 7 | 13 | 2 | 64 | 1/345,188 |
*The mutant library consists of 5,771 cryopreserved male progeny from ENU-mutagenized fish. †Due to the compact medaka genome architecture, multiple exons can often be amplified and sequenced from a single amplicon. ‡Determined by counting all bases in the resequencing reads that were read with phred quality >20.