Table 1.
A list of primer and probe sequences for the genes evaluated
| Gene | Forward | Reverse | Probe |
| ADAMTS5 | TGGGTTCCCAAATATGCAG | CTGTCCCATCCGTCACCT | CTGGGAGA |
| 1AGC1 | GGGACCTGTGTGAGATCGAC | GTAACAGTGGCCCTGGAACT | AGGAGCTG |
| BGN | CAGAACAACGACATCTCAGAGC | TCACCAGGACGAGAGCGTA | CTCCACCA |
| COL1A2 | CTATCAATGGTGGTACCCAGTTT | TGTTTTGAGAGGCATGGTTG | GCCTGCTG |
| COL2A1 | CTGGTGAACCTGGACGAGAG | ACCACGATCACCCTTGACTC | CCTCCTGG |
| COL3A1 | GGATGGTGGCTTCCAGTTT | CCAGCTGGACATCGAGGA | GCTGCCTG |
| COL5A1 | AACCTGTCGGATGGCAAGT | CAGTCCAAGATCAAGGTGACAT | CAGCATCC |
| COL9A3 | CGAGGTGCCTCAGGTGAC | ACCCAGCTCTCCTTTGTCC | GAGACCAG |
| CSPG2 | TGGATGGTTTTAATACGTTCAGG | GCCGTAGTCACACGTCTCTG | CTGCCTTC |
| CTSB | CGGCCTTCACCGTGTACT | GTGACGTGCTGGTACACTCC | CTTCCTGC |
| CTSD | GGTCCACATGGAGCAGGT | TATGAGGGAGGTGCCTGTGT | TGGGCAGC |
| DCN | CGCTGTCAGTGCCATCTC | GGGGGAAGATCTTTTGGTACTT | TCCAGTGT |
| GAPDH | CTGGGGCTCACTTGAAAGG | CAAACATGGGGGCATCAG | CTGCTCCT |
| Genomic | AACCCTCAAAGATGAGGTTTAGC | ACTCTGGGATCACGCATGT | CTGCCTTC |
| LUM | ACCTGGAAATTCTTTTAATGTATCATC | CGGTATGTTTTTAAGCTTATTGTAGGA | TGCTGGAG |
| MMP13 | CCGCGACCTTATCTTCATCT | AACCTTCCAGAATGTCATAACCA | AGAGGCAG |
| RPL13A | CTGCCCCACAAGACCAAG | GGGATCCCATCAAACACCT | CCAGGCTG |
| SDHA | GGTGGCACTTCTACGACACC | ATGTAGTGGATGGCGTCCTG | CTGGCTGG |
| TBP | TCCACAGCCTATCCAGAACA | CTGCTGCTGTTGTCTCTGCT | CTGGAGGA |
| TIMP1 | TGCATCCTGCTGTTGCTG | AACTTGGCCCTGATGACG | CCCAGCAG |
| TIMP2 | ATGGGCTGTGAGTGCAAGAT | CACTCATCCGGAGACGAGAT | CTGCCCCA |
| TIMP4 | GCAGAGAGAAAGTCTGAATCATCA | GGCACTGTATAGCAGGTGGTAA | TGTGGCTG |
| TNC | TGGATGGGACAGTCAAGGA | GCTCAGCTCTGCCAGGTTA | CCACCTCC |
| VIM | TACAGGAAGCTGCTGGAAGG | CCTCAGGTTCAGGGAAGAAA | GAGCAGGA |
ADAMTS5, ADAM metallopeptidase with thrombospondin type 1 motif, 5; AGC1, aggrecan; BGN, biglycan; COL1A2, type I collagen, alpha two chain; COL2A1, type II collagen alpha 1 chain; COL3A1, type III collagen alpha 1 chain; COL5A1, type V collagen alpha 1 chain; COL9A3, type IX collagen alpha 3 chain; CSPG2, chondroitin sulphate proteoglycan 2; CTSB, cathepsin B; CTSD, cathepsin D; DCN, decorin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Genomic, Genomic DNA, LUM, lumican;MMP13, matrix metalloproteinase 13; RPL13A, ribosomal protein L13a; SDHA, succinate dehydrogenase complex, subunit A; TBP, TATA box binding protein; TIMP1, tissue inhibitor of metalloproteinase 1; TIMP2, tissue inhibitor of metalloproteinase 2; TIMP4, tissue inhibitor of metalloproteinase 4; TNC, Tenascin C; VIM, vimentin.