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Modulation of LTB4 production by 1,25(OH)2D3 and TGF-β in osteoarthritis osteoblasts. Confluent cells were incubated for their last 48 hours in Ham's F12/DMEM medium containing 2% fetal bovine serum in the presence or absence of 50 nM 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), 10 ng/ml transforming growth factor-β (TGF-β), or both. Leukotriene B4 (LTB4) was measured in conditioned medium with the use of a very selective ELISA. Values are means ± SEM for n = 4 samples for all groups.
