Table 1.
Evaluation of phenotypic markers of normal and osteoarthritis (OA) subchondral osteoblasts
| Source | Alkaline phosphatase (nmol/mg protein/30 min) | Osteocalcin (ng/mg protein/48 hours) | Collagen type I (ng/mg protein/48 hours) |
| Normal (n = 11) | 624.7 ± 88.8 | 176.6 ± 24.7 | 285.3 ± 17.1 |
| OA (n = 26) | 1333.1 ± 215.7 | 264.0 ± 20.7 | 370.4 ± 8.1 |
| p < 0.005 | p < 0.05 | p < 0.015 |
Confluent osteoblasts were incubated for their last 2 days of culture in Ham's F12/DMEM medium containing 2% charcoal-treated fetal bovine serum and 50 nM 1,25-dihydroxyvitamin D3. Values are means ± SEM. The statistical analysis compared OA values with their respective normal values.