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. 2006 Dec 22;8(6):R188. doi: 10.1186/ar2101

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Copyright © 2006 Truong et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Representative gene expression as determined by semi-quantitative reverse transcription-polymerase chain reaction (PCR) using total RNA extracted from intertrochanteric trabecular bone. Target genes included alkaline phosphatase (ALP) (434 bp), osteocalcin (OCN) (159 bp), osteopontin (OPN) (472 bp), insulin-like growth factor (IGF)-I (344 bp), IGF-II (263 bp), transforming growth factor-β1 (TGF-β1) (224 bp), COL1A1 (339 bp), COL1A2 (778 bp), and the housekeeping gene GAPDH (415 bp). Specimens were obtained from a 60-year-old female (F 60) and a 59-year-old male (M 59) undergoing total hip replacement for primary osteoarthritis (OA). The control specimens were obtained at autopsy from a 61-year-old female (F 61) and a 60-year-old male (M 60) without any bone-related disease. PCR products representing each mRNA species were visualised on SYBR Gold^®-stained 2% agarose gels. COL1A, collagen type I alpha chain; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.