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. Author manuscript; available in PMC: 2007 Feb 8.
Published in final edited form as: Biochemistry. 2007 Feb 6;46(5):1368–1379. doi: 10.1021/bi061949m

Figure 4. Alteration of NH2-terminal charge affects nearby and distal conformation in chimeric TnT model molecules.

Figure 4

(A) ELISA epitope affinity titration curves of mAb 3E4 demonstrate that the Acidic and Basic chimeric TnTs exhibit a conformational difference at the 3E4 epitope near the NH2-terminal variation site, as defined by the difference in antibody concentrations required for 50% maximal binding. (B) Titration curves of mAb CT3 demonstrate that the Acidic and Basic chimeric TnTs and the native adult mouse cardiac TnT exhibit different conformation at the CT3 epitope in the central region (Fig. 1A), indicating remote conformational effect of the N-terminal variation.