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. Author manuscript; available in PMC: 2007 Feb 8.
Published in final edited form as: Biochemistry. 2007 Feb 6;46(5):1368–1379. doi: 10.1021/bi061949m

Figure 8. Preserved myofilament incorporation and sustained in vivo function of the TnT core structure.

Figure 8

(A) SDS-PAGE and Western blot using the CT3 mAb demonstrates the high level expression of the cardiac TnT core in the heart of adult McTnT-ND72 transgenic mice. mAb TnI-1 Western blot shows that McTnT-ND72 co-migrates with the endogenous cardiac TnI. (B) Coomassie Blue staining of SDS-PAGE gels demonstrates the stoichiometric recovery of tertiary Tn complex from wild type and Tg McTnT-ND72 hearts using TnI-1 mAb immunoaffinity chromatography. As described above, the co-migration of TnT-ND72 with TnI formed a fused band in the Tn sample from the Tg McTnT-ND72 hearts. In addition to the stoichiometric recovery of Tn subunits, the TnI affinity column also recovered Tm, indicating the native biological activity of the Tn complex. High percentages of the exogenous cardiac TnT in the total cardiac tissue of transgenic mouse heart (solid bars) were recovered in the isolated Tn complex (open bars), indicating that McTnT-ND72 and Embryonic TnT both effectively incorporate into the myofilament in vivo.