Figure 1.

A multiple-tissue expression array (CLONTECH) of polyA RNA from 76 human tissues and cell lines probed with human cDNAs for α-, β-1, β-2, and γ-adducins. α- and γ-adducins show expression in all samples whereas β-1 and β-2 show restricted expression, mainly in brain and hematopoietic tissues. Probes for β-1 and β-2 were generated by PCR to encompass their distinct carboxy termini and 3′UTRs (β-1, exons 14–17; β2, exon 13b). Subcloned PCR products were labeled by random priming with ³²P-dCTP.