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H-NS-dependent stability and turnover of StpA. The relative stability of StpA was measured after protein synthesis was inhibited as described in the text. StpA stability was measured in the following strains: (A) JGJ212 (hns+, stpA+); (B) JGJ213 [hns+, stpA(F21C)]; (C) JGJ214 (hns, stpA+); and (D) JGJ215 [hns, stpA(F21C)]. (E) Quantitative determinations of StpA from the different experiments in A–D plotted as a function of time.
