Figure 3.

Stably maintained pSC1 adopts a nucleosomal structure that can be disrupted by butyrate treatment. Nucleosome positioning on transiently (lanes 1–6) or stably maintained (lanes 7–12) pSC1, isolated either from untreated or butyrate-treated cells, was determined by treating purified nuclei with micrococcal nuclease (MNase, as indicated), and digested DNA was detected by Southern blot. Molecular weight markers are indicated on the left, and the nucleosomal ladder is indicated on the right.