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μ+L− bursal cells in the neonatal bursa of RCAS-Tμ-infected chicks are proliferating in epithelial buds. (A and B) Frozen sections of bursal tissue stained with anti-ChB6 from neonatal chicks infected as day 3 embryos with RCAS-Tμ (A) or RCAS (B). The RCAS-Tμ bursa shown contained 18.5% μ+L− cells. (C and D) Histograms from 10,000 μ+L+ (C) or μ+L− (D) cells purified by FACS cell sorting and analyzed for cellular DNA content by propidium iodide staining.
