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. 1999 Sep 14;96(19):10812–10817. doi: 10.1073/pnas.96.19.10812

Figure 1.

Figure 1

Targeted disruption of the ceruloplasmin gene. (A) Cp locus, targeting vector and predicted recombinant allele. The 5′ flanking probe used for Southern analysis is shown. Restriction sites: E, EcoRV; B, BglII. (B) PCR products from tail genomic DNA corresponding to a 0.5-kb fragment (made by using primers a and b) from exon 16 to exon 17 in Cp+/+ alleles and a 1.0-kb fragment (made by using primers c and d) from exon 16 to the Neo cassette in Cp−/− mice. (C) Immunoblot analysis of ceruloplasmin in serum from Cp+/+, Cp+/−, and Cp−/− mice. (D) Ferroxidase activity in serum of Cp+/+, Cp+/−, and Cp−/− mice. Results are expressed as means ± standard deviations; ∗, P < 0.001.