Figure 4.

(A) ⁵⁹Fe uptake in the livers of control and transferrin-saturated (Tf-Fe) 16-wk-old Cp^+/+ and Cp^−/− mice. Results are expressed as means ± standard deviations, n = 6 per group. (B) Analysis of serum ⁵⁹Fe in transferrin saturated Cp^+/+ and Cp^−/− mice revealed a statistically significant difference 96 hr after ⁵⁹Fe injection (∗, P < 0.001). Results are expressed as means ± standard deviations, n = 4 per group. Subsequent infusion of ceruloplasmin (Cp) or apoceruloplasmin (apoCp) as 6 μg/100 μl of circulating blood volume in these mice reveals a statistically significant increase in ⁵⁹Fe in the serum of Cp^−/− mice (∗, P < 0.001).