Table 2.
Substitutions observed among guppy LWS opsins, and comparable mutants made in bovine RH1 and human LWS.
| Observed Substitutions Among Guppy Opsins1 | Comparable Mutants Made in Bovine RH12 [48] | Transducin Activity Relative to Wildtype RH1 [48] |
| S256C | S256A | 60% |
| K261N | K261L | 98% |
| R264K | K264L | 72% |
| H247R, A248D | K247T, E248Q | 87% |
| K261N, R264K | K261L, K264L | 61% |
| E263Q, R264K | E263Q, K264L | 110% |
| Observed Substitutions Among Guppy Opsins1 | Comparable Mutants Made in Human LWS2 [45, 46] | Effect on LWS Spectral Tuning LWS [45, 46] |
| I230T | I230T | 2–3 nm |
| A233G | A233S | 2–3 nm |
| Y277F | Y277F | 6–10 nm |
| T285A | T285A | 10–16 nm |
| Y277F, T285A | Y277F, T285A | 18–27 nm |
1 Substitutions observed among guppy LWS opsins; refer to text and Fig. 4 for more details
2 Mutants created in bovine RH1 and human LWS by site-directed mutagenesis