Fig. 3.

Construction of a hybrid protein carrying TM4 Tmp mt3. A. Schematic representation of TM4 gp17 Tmp and MSMEG3721 showing the position of mt3. A hybrid protein was created by replacing mt3 in MSMEG3721 with the motif present in the Tmp of TM4. B. E. coli cell extracts expressing the indicated proteins were separated by SDS-PAGE (left panel) or separated and analysed in a zymogram for peptidoglycan-hydrolysing activity (right panel). Lanes 1 and 4, extract of E. coli cells transformed with pET-28a (empty vector); lanes 2 and 5, E. coli cell extracts overexpressing MSMEG3721; lanes 3 and 6, E. coli cell extracts overexpressing the hybrid protein.