Table 1.
Minimal inhibitory concentration (MIC) for vancomycin of wt and mutant strains.
| Strain | Vancomycin MIC (μg ml−1) ± SD | R factor* |
|---|---|---|
| Wild type | 2.43 ± 0.04 | 1 |
| MSMEG3721:: res-hyg-res | 5.26 ± 0.20 | 2.2 |
| MSMEG0642:: res-hyg-res | 4.83 ± 0.09 | 2.0 |
| MSMEG0642Δ/3721:: res-hyg-res | 4.99 ± 0.18 | 2.1 |
| MSMEG6076:: res-hyg-res | 12.41 ± 0.70 | 5.1 |
| wt (pPG1) | 4.64 ± 0.04 | 1 |
| MSMEG0642Δ/3721:: res-hyg-res (pPG1) | 8.75 ± 0.03 | 1.9 |
| MSMEG0642Δ/3721:: res-hyg-res (pMP8) | 4.51 ± 0.09 | 1 |
| MSMEG0642Δ/3721:: res-hyg-res (pMP9) | 4.72 ± 0.16 | 1 |
Experiments were done by triplicate and the mean for the MIC and the SD are shown.
*
R factor represents the resistance factor of each strain compared with M. smegmatis mc2155 (wild type).